@Article{Mariscal-MuñozCTBHMLS:2016:OsDiEn,
author = "Mariscal-Muñoz, Eduardo and Costa, Carlos A. S. and Tavares,
Hewerson S. and Bianchi, Jonas and Hebling, Josmeri and Machado,
Jo{\~a}o Paulo Barros and Lerner, Ulf H. and Souza, Pedro P. C.",
affiliation = "{Universidade Estadual Paulista (UNESP)} and {Universidade
Estadual Paulista (UNESP)} and {Universidade Estadual Paulista
(UNESP)} and {Universidade Estadual Paulista (UNESP)} and
{Universidade Estadual Paulista (UNESP)} and {Instituto Nacional
de Pesquisas Espaciais (INPE)} and {Umeå University} and
{Universidade Estadual Paulista (UNESP)}",
title = "Osteoblast differentiation is enhanced by a nano-to-micro hybrid
titanium surface created by Yb:YAG laser irradiation",
journal = "Clinical Oral Investigations",
year = "2016",
volume = "20",
number = "3",
pages = "503--511",
month = "Apr.",
keywords = "Bone, Laser, Osteoblast, Titanium implants.",
abstract = "Objectives: The aim of this study was to analyze the capacity of a
new modified laser surface to stimulate calvarial osteoblasts
isolated from neonatal mouse bones to differentiate and form
mineralized nodules. Methods: Titanium discs were subjectezd or
not to laser irradiation according to specific parameters and
characterized. Osteoblasts isolated from neonatal mouse calvaria
were cultured over the discs, and the capacity of these cells to
proliferate (MTT assay), form mineralized nodules (Alizarin red
assay), and enhance alkaline phosphatase activity (ALPase
activity) was analyzed. Real-time PCR was used for quantification
of gene expression. Results: Laser-irradiated titanium discs (L)
presented a rough nano-to-micrometric oxidized surface contrasting
with the smooth pattern on polished discs (P). The Ra on the
micrometric level increased from 0.32 ± 0.01 \μm on P
surfaces to 10.57 ± 0.39 \μm on L surfaces. When compared
with P, L promoted changes in osteoblast morphology, increased
mineralized nodule formation in osteoblasts cultured on the
surfaces for 14 days, and enhanced ALPase activity at days 7 and
14. Transcription factors triggering osteoblast differentiation
(Runx2 and Sp7) and genes encoding the bone extracellular matrix
proteins collagen type-1 (Col1a1), osteopontin (Spp1), and
osteocalcin (Bglap) were upregulated in cells on L surfaces
compared with those on P surfaces at days 114. Conclusion: Laser
treatment of titanium surfaces created a rough surface that
stimulated osteoblast differentiation. Clinical relevance: Laser
treatment of titanium generates a reproducible and efficient
surface triggering osteoblast differentiation that can be of
importance for osteointegration.",
doi = "10.1007/s00784-015-1533-1",
url = "http://dx.doi.org/10.1007/s00784-015-1533-1",
issn = "1432-6981",
language = "en",
targetfile = "mariscal_osteoblast.pdf",
urlaccessdate = "04 maio 2024"
}